Interactions of proteins with graphene and carbon nanotubes
Glucose oxidase (GOx) is an enzyme which catalyze oxidation of β-D-glucose by molecular oxygen. Since 60ties the medical industry is employing this enzyme for construction of glucose biosensors. By incorporating nanomaterials, it is possible to achieve enhanced sensitivity, improved response time and smaller size. The direct electrochemistry of enzyme refers to the direct electron communication between the electrode and the active center of the enzyme without the participation of mediators or other reagents. However, the realization of direct electrochemistry of redox enzymes on common electrodes is very difficult because the active centers of most redox enzymes are located deeply in a hydrophobic cavity of the molecule (e.g. FAD – flavin cofactor in case of GOx). It was experimentally confirmed that the modified electrode surfaces involving graphene or carbon nanotubes can increase electron-transfer-rate. Proteins can also be used for construction of nanoelectronics, photovoltaics and other chemical/biological sensors so determination of contacts of proteins with modified electrode materials is of great importance.
ERNEST = European Research Network on Signal Transduction
The main scientific objective of the Action is to develop a common, comprehensive and holistic map of signal transduction that will advance development of pathway-specific chemical modulators. This unique and innovative goal will be realised by linking of a diverse group of researchers in the field through the networking activities funded by COST.
Our service GPCRM is completely reshaped, much faster, and user friendly. Now, it contains 3 main routes: Quick path (default), Long path, and High similarity (the fastest) for homology modeling of GPCRs. Currently, the service contains over 90 template structures. The updated version was recently published in NAR 2018, W1.